DOT Urine Specimen Collector Training will take approximately 10-hours to complete, including the mandatory mock collections. Training is user-friendly and designed by leading drug testing expert and attorney Andrew David Easler, Esq., and is made for a wide audience, including new urine specimen collectors, DOT-regulated employers, and third-party testing sites that must adhere to rigorous drug screening compliance, such as, but not limited to, urgent care centers, chiropractors, and other healthcare facilities who wish to conduct DOT-regulated drug screening.
Training provides learners with techniques and regulations surrounding drug testing, ensuring training complies with the Department of Transportation's 49 CFR Part 40.33 regulations, which also requires hands-on learning via virtual mock collections. These include the five dry mock collections that cover the most common scenarios collectors are likely to encounter while performing urine specimen collections, providing a practical and real-world perspective to the training.
We provide an easy-to-understand set of training materials and an online course that provides the practical aspects of the collection and allows learners to absorb and reinforce theoretical knowledge at their own pace, with training being designed to marry theoretical understanding with practical application, preparing learners for real-world tasks.
In addition to the initial qualification to collect urine under DOT protocols and chain of custody, qualified collectors must enroll in mandatory recertification every five years. This recertification training ensures that all qualified urine specimen collectors remain up-to-date, enhancing the accuracy and integrity of drug testing procedures over time and reducing fatal flaws.
Register for DOT Urine Specimen Collector Training using our online platform that features engaging virtual mock collections, or if you prefer a more hands-on approach, consider our on-site training option. For organizations desiring to incorporate this course into their in-house training, we offer licensable courseware that can be smoothly integrated into any third-party learning system.
$850.00 Add more credits to save Maximum Discounts Unlocked
Add 2 more credits to save 12%
Maximum Discounts Unlocked
Accepted Payment Methods
Not all payment methods are available to all users. Minimums and terms may apply.
Published August 8, 2025
Andrew David Easler, Esq.
Course Author
Melissa Wheeler
Course Instructor
In addition to initial online, self-paced proficiency training, this course includes live, instructor-led mock collection sessions. A $150 cancellation/no-show fee per learner applies once the session is scheduled.
DOT Urine Specimen Collector Training guides learners through the Department of Transportation's 49 CFR Part 40.33 requirements so they can adequately perform DOT-regulated urine specimen collections.
As an employer or third-party testing site that performs drug testing for DOT-regulated sectors, such training is essential for maintaining a safe and productive environment and ensuring your organization's stringent adherence to all drug and alcohol testing regulations under 40 CFR Part 40. This adherence to drug screening compliance is fundamental in reducing potential lawsuits resulting from compliance failures.
DOT guidelines dictate strict adherence to several safety and security protocols to complete a urine specimen collection. Participants in this 10-hour course will learn essential topics such as the DOT collection process, handling procedures, and regulatory requirements, including industry best practices for drug and alcohol testing for employees regulated under the following agencies:
A DOT Urine Specimen Collector plays a crucial role in the drug testing process for DOT-regulated industries, ensuring that the collection and handling of urine specimens follow DOT regulations. Becoming qualified with a reputable trainer is essential for anyone looking to work in this field as it demonstrates a high level of competency and expertise in DOT urine specimen collection procedures.
Training materials ship directly to the learner's home or work and include a valuable guide to federal regulations on drug and alcohol testing and virtual mock demonstrations (mocks) conducted by webcam. Collectors appropriately trained by an experienced and qualified instructor can minimize legal risks and foster a workplace culture of safety and compliance.
DOT training for FMCSA, FAA, FTA, FRA, USCG, and PHMSA that meets or exceeds federal requirements. Accepted in all 50 states and anywhere DOT rules apply.
Every course is reviewed by attorneys and subject matter experts for accuracy, so you get training you can show employers, clients, and auditors with confidence.
Questions about your course? Our US-based trainers help learners, managers, and supervisors by phone or email during business hours and respond quickly.
Train on any computer, phone, or tablet. Courses stay open until you finish, so learners can work at their own pace with no deadline.
Buy course credits now and assign them when you need them. Credits never expire until a learner accepts an invitation, and volume discounts apply at checkout.
Learners download their certificate as soon as they pass the exam. Managers can view and download certificates for their whole team from one account.
Buy two or more credits and get a free manager account. Invite employees, track completions, and keep training records in one place.
Learners can start their course the moment they register or accept an invitation. Managers get their credits right away and can enroll employees with no waiting.
Go beyond the course with books, guides, and free videos and posts on YouTube, LinkedIn, TikTok, and more covering drug testing, workplace safety, and compliance.
Take any collector course and get a free first year of GoBig on MyDrugTesting.com, so TPAs, labs, MROs, and employers can find you. Visibility only, leads not guaranteed.
Learners will gain a solid understanding of the DOT's 49 CFR Part 40 guidelines for urine specimen collection and should be able to interpret and apply these guidelines accurately throughout the collection process.
Learners will learn and practice the proper techniques for urine specimen collection, ensuring accurate and reliable results. They will become skilled in the entire collection process, including preparing the collection area, ensuring privacy and security, following chain of custody procedures, and documenting relevant information accurately.
Learners will be familiarized with the various resources available for drug screening, such as collection kits, testing equipment, and documentation forms. They will learn how to effectively use these resources to ensure proper handling, storage, and transportation of specimens, maintaining the integrity of the testing process.
Learners will develop a solid understanding of industry-specific vocabulary, acronyms, and jargon commonly used in the field of urine specimen collection. They will be able to communicate effectively with colleagues, clients, and other stakeholders, using appropriate terminology and abbreviations.
Learners will learn and understand the importance of chain of custody procedures in urine specimen collection. They will learn how to maintain a secure and documented process from when the specimen is collected until it reaches the laboratory for testing. This includes proper labeling, sealing, handling, and documentation to ensure legal defensibility and accuracy.
Learners will be educated on the importance of ethical conduct, confidentiality, and compliance with relevant regulations and legal requirements in urine specimen collection. They will learn to recognize potential ethical dilemmas and handle them appropriately while maintaining professionalism and integrity.
21 modules · 1 exam · 1 assignment
Module 1
DOT versus Non-DOT Drug Testing
Module 2
Training Regulations
Module 3
49 CFR Part 40
Module 4
DOT Urine Specimen Collection Guidelines
Module 5
Qualification Training
Module 6
Collecting the Specimen
Module 7
Direct Observer vs. Monitor Review
Module 8
Flaws and Errors in Collection
Module 9
Best Practices
Module 10
Supporting Materials (Welcome Packet) Collection Checklist
Module 11
Supporting Materials (Welcome Packet) Collection Site Checklist
Module 12
Supporting Materials (Welcome Packet) Federal Drug Testing Custody and Control Form
Module 13
Supporting Materials (Welcome Packet) Memorandum to File
Module 14
Supporting Materials (Welcome Packet) DOT's 10 Steps to Collection Site Security and Integrity
Module 15
Supporting Materials (Welcome Packet) DOT's 10 Steps to Collection Site Security (Spanish)
Module 16
Supporting Materials (Welcome Packet) DOT's Direct Observation Procedures
Module 17
Supporting Materials (Welcome Packet) Pre-Collection Checklist
Module 18
Supporting Materials (Welcome Packet) Records Access Policy
Module 19
Supporting Materials (Welcome Packet) Service Authorization
Module 20
Supporting Materials (Welcome Packet) Water Log
Exam
DOT Urine Specimen Collector Exam
Module 21
Schedule Mock Collections
Assignment
Upload Mock Collections
Built to meet training requirements in 45 states. Not covered: Ohio, Oklahoma, Oregon, Pennsylvania, Rhode Island.
Hardware
Software
Other requirements
Qualification training and five consecutive error-free mock collections under 49 CFR 40.33. Both must be completed before you perform any DOT collections.
All steps of a correct collection and the Custody and Control Form. It also covers problem collections like shy bladder and tampering, fatal and correctable flaws, and protecting the privacy of the Donor and the security of the specimen.
Two uneventful collections, one with too little urine, one with a temperature out of range, and one where the Donor refuses to sign the CCF and initial the bottle seal. All five must be error-free and in a row.
A qualified urine collector who has performed DOT collections for at least a year, has trained collectors for at least a year, or has completed a urine train the trainer course. They can observe in person or by live video with real-time interaction.
At least every five years. Refresher training must repeat the full qualification training and the five error-free mock collections.
If you make a mistake that causes a test to be cancelled, you must complete error correction training within 30 days of being notified. It includes three error-free mock collections. Our DOT Urine Specimen Collector Error Correction Training covers it.
90 to 100 °F, checked within 4 minutes of the Donor handing over the specimen. A reading outside that range calls for a directly observed collection.
The Donor may drink up to 40 ounces of fluid over up to three hours. If there is still not enough urine after three hours, the collector stops the collection and notifies the DER.
No. The collector documents what happened and tells the DER. The employer makes the final refusal determination.
Yes. DOT oral fluid collection has its own qualification rules under 49 CFR 40.35. Take DOT Oral Fluid Collector Training.
Documentation showing you meet all training requirements. You must provide it on request to DOT agencies, employers, and C/TPAs that use or plan to use your services.
Follower and subscriber counts may not reflect the most up-to-date information, as these change frequently. Visit each social channel directly for the current follower and subscriber count.
We'll send you a short digest of new updates for the agencies you follow.
Once confirmed, you'll be able to select agencies for regulatory alerts.
Frequently Asked Questions
No FAQs are available for this selection.
More FAQNo agency updates are available for this selection.
Explore moreYour cart is ready for a course.
Attorney Trusted® Training
Add more credits to save
Maximum Discounts Unlocked
Search the catalog by agency, state or topic, and choose Add to Cart when you find the right training.
Find your course Browse bundlesCourses and bundles are paid for in separate orders. You choose which to pay for first on the next page.
Payment details are confirmed in the next step.